Jeffrey Petty
Professor, Chemistry
- Email: jeff.petty@furman.edu
- Phone: 864.294.2689
- Office: Plyler 244F, Townes Science Center
Dr. Jeffrey Petty is interested in the interface of physical chemistry, biochemistry, and inorganic chemistry. He trained with Brad Moore in the spectroscopy of atmospherically significant free radicals. He then continued his studies with Dick Keller in the area of ultrasensitive, laser-based biomolecular detection. Dr. Petty continues his studies with his students and colleagues in the area of silver cluster-DNA interactions.
Transient Absorption Infrared Spectroscopy of Atmospheric Radicals
Spectroscopy and Structure of DNA-Bound Silver Clusters
Education
- Ph.D., University of California, Berkeley
- B.S., Furman University
Research
Our studies revolve around the small sizes of silver clusters. These molecular metals have discrete electronic states, localized optical transitions, and strong fluorescence. DNA strands serve two purposes: (1) Their nucleobases coordinate specific species, and specific sequences yield chromophores with spectra that span the visible and near-infrared spectral regions; (2) They direct cluster transformations via changes in their secondary structure. Our studies revolve around pairs of chromophores that interconvert via hybridization. These conjugate cluster-DNA chromophores are innovative because of two features – hybridization switches on fluorescence and two-color laser excitation enhances the overall cluster emission. Mass spectrometry and X-ray absorption spectroscopy establish the basis for the fluorescence switching. Changes in the stoichiometry, charge, and structure of pairs of dim/bright chromophores are investigated. Fluorescence correlation spectroscopy evaluates the basis for the two-color enhanced fluorescence. These two sets of studies will provide the foundation to develop a new class of optical sensors. These sensors will be used to detect low abundance microRNA sequences that are relevant for early cancer intervention.
Publications
- Tug-of-War between DNA Chelation and Silver Agglomeration in DNA–Silver Cluster Chromophores. J. T. Petty, D. Lewis, S. Carnahan, D. Kim and C. Couch. The Journal of Physical Chemistry B 2022 Vol. 126 Issue 21 Pages 3822-3830. DOI: 10.1021/acs.jpcb.2c01054
- Interrupted DNA and Slow Silver Cluster Luminescence. D. Lewis, C. Setzler, P. M. Goodwin, K. Thomas, M. Branham, C. A. Arrington and J. T. Petty. The Journal of Physical Chemistry C 2023 Vol. 127 Issue 22 Pages 10574-10584. DOI: 10.1021/acs.jpcc.3c01050
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Petty, J. T.; Sergev, O. O.; Ganguly, M.; Rankine, I. J.; Chevrier, D. M.; Zhang, P. A Segregated, Partially Oxidized, and Compact Ag10 Cluster within an Encapsulating DNA Host. J. Am. Chem. Soc., 2016, 138 (10), 3469-3477.
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Petty, J. T.; Sergev, O. O.; Kantor, A. G.; Rankine, I. J.; Ganguly, M.; David, F. D.; Wheeler, S. K.; Wheeler, J. F. Ten-Atom Silver Cluster Signaling and Tempering DNA Hybridization. Anal. Chem. 2015, 87(10), 5302-5309.
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Ganguly, M.; Bradsher, C.; Goodwin, P.; Petty, J. T. DNA-Directed Fluorescence Switching of Silver Clusters. J. Phys. Chem. C, 2015, 119 (49), 27829-27837.
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Petty, J. T.; Nicholson, D. A.; Sergev, O. O.; Graham, S. K. Near-infrared silver cluster optically signaling oligonucleotide hybridization and assembling two DNA hosts. Anal. Chem. 2014, 86, 9220-8.
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Petty, J. T.; Giri, B.; Miller, I. C.; Nicholson, D. A.; Sergev, O. O.; Banks, T. M.; Story, S. P. Silver clusters as both chromophoric reporters and DNA ligands. Anal. Chem. 2013, 85, 2183-90.
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Petty, J. T.; Sergev, O. O.; Nicholson, D. A.; Goodwin, P. M.; Giri, B.; McMullan, D. R. A silver cluster-DNA equilibrium. Anal. Chem. 2013, 85, 9868-76.
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Petty, J. T.; Story, S. P.; Hsiang, J.; Dickson, R. M. DNA-Templated Molecular Silver Fluorophores. J. Phys. Chem. Lett. 2013, 4, 1148-1155.
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Petty, J. T.; Story, S. P.; Juarez, S.; Votto, S. S.; Herbst, A. G.; Degtyareva, N. N.; Sengupta, B. Optical sensing by transforming chromophoric silver clusters in DNA nanoreactors. Anal. Chem. 2012, 84, 356-64.
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Degtyareva, N. N.; Barber, C. A.; Reddish, M. J.; Petty, J. T. Sequence length dictates repeated CAG folding in three-way junctions. Biochemistry 2011, 50, 458-65.
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Degtyareva, N. N.; Petty, J. T. Non-B conformations of CAG repeats using 2-aminopurine. Meth. Enzymol. 2011, 492, 213-31.
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Petty, J. T.; Fan, C.; Story, S. P.; Sengupta, B.; Sartin, M.; Hsiang, J.; Perry, J. W.; Dickson, R. M. Optically enhanced, near-IR, silver cluster emission altered by single base changes in the DNA template. J. Phys. Chem. B 2011, 115, 7996-8003.
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Petty, J. T.; Sengupta, B.; Story, S. P.; Degtyareva, N. N. DNA sensing by amplifying the number of near-infrared emitting, oligonucleotide-encapsulated silver clusters. Anal. Chem. 2011, 83, 5957-64.
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Petty, J. T. Book Review of Advanced Fluorescence Reporters in Chemistry and Biology II: Molecular Constructions, Polymers and Nanoparticles. J. Am. Chem. Soc. 2011, 133(26), 10323-10323.
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Degtyareva, N. N.; Barber, C. A.; Sengupta, B.; Petty, J. T. Context dependence of trinucleotide repeat structures. Biochemistry 2010, 49, 3024-30.
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Loo, K. M.; Degtyareva, N. N.; Park, J.; Sengupta, B.; Reddish, M. J.; Rogers, C. C.; Bryant, A. R.; Petty, J. T. Ag+-mediated assembly of 5'-guanosine monophosphate. J. Phys. Chem. B 2010, 114, 4320-6.
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Petty, J. T.; Fan, C.; Story, S. P.; Sengupta, B.; Iyer, A. S. J.; Prudowsky, Z. D.; Dickson, R. M. DNA Encapsulation of Ten Silver Atoms Produces a Bright, Modulatable, Near Infrared-Emitting Cluster. J. Phys. Chem. Lett. 2010, 1, 2524-2529.
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Degtyareva, N. N.; Reddish, M. J.; Sengupta, B.; Petty, J. T. Structural studies of a trinucleotide repeat sequence using 2-aminopurine. Biochemistry 2009, 48, 2340-6.
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Sengupta, B.; Springer, K.; Buckman, J. G.; Story, S. P.; Abe, O. H.; Hasan, Z. W.; Prudowsky, Z. D.; Rudisill, S. E.; Degtyareva, N. N.; Petty, J. T. DNA Templates for Fluorescent Silver Clusters and I-Motif Folding. J. Phys. Chem. C 2009, 113, 19518-19524.
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Sengupta, B.; Ritchie, C. M.; Buckman, J. G.; Johnsen, K. R.; Goodwin, P. M.; Petty, J. T. Base-Directed Formation of Fluorescent Silver Clusters. J. Phys. Chem. C 2008, 112, 18776-18782.
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Degtyareva, N. N.; Fresia, M. J.; Petty, J. T. DNA conformational effects on the interaction of netropsin with A-tract sequences. Biochemistry 2007, 46, 15136-43.
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Degtyareva, N. N.; Wallace, B. D.; Bryant, A. R.; Loo, K. M.; Petty, J. T. Hydration changes accompanying the binding of minor groove ligands with DNA. Biophys. J. 2007, 92, 959-65.
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Ritchie, C. M.; Johnsen, K. R.; Kiser, J. R.; Antoku, Y.; Dickson, R. M.; Petty, J. T. Ag Nanocluster Formation Using a Cytosine Oligonucleotide Template. J. Phys. Chem. C 2007, 111, 175-181.
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Kiser, J. R.; Monk, R. W.; Smalls, R. L.; Petty, J. T. Hydration changes in the association of Hoechst 33258 with DNA. Biochemistry 2005, 44, 16988-97.
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Petty, J. T.; Zheng, J.; Hud, N. V.; Dickson, R. M. DNA-templated Ag nanocluster formation. J. Am. Chem. Soc. 2004, 126, 5207-12.
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Zheng, J.; Petty, J. T.; Dickson, R. M. High quantum yield blue emission from water-soluble Au8 nanodots. J. Am. Chem. Soc. 2003, 125, 7780-1.
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Bordelon, J. A.; Feierabend, K. J.; Siddiqui, S. A.; Wright, L. L.; Petty, J. T. Viscometry and Atomic Force Microscopy Studies of the Interactions of a Dimeric Cyanine Dye with DNA. J. Phys. Chem. B 2002, 106, 4838-4843.
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Barker, K. D.; Benoit, B. R.; Bordelon, J. A.; Davis, R. J.; Delmas, A. S.; Mytykh, O. V.; Petty, J. T.; Wheeler, J. F.; Kane-Maguire, N. Intercalative binding and photoredox behavior of Cr(phen)2(dppz)]3+ with B-DNA. Inorg. Chim. Acta 2001, 322, 74-78.
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Petty, J. T.; Bordelon, J. A.; Robertson, M. E. Thermodynamic Characterization of the Association of Cyanine Dyes with DNA. J. Phys. Chem. B 2000, 104, 7221-7227.
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Knight, L. B.; King, G. M.; Petty, J. T.; Matsushita, M.; Momose, T.; Shida, T. Electron spin resonance studies of the methane radical cations (12,13CH+4, 12,13CDH+3, 12CD2H+2,12CD3H+, 12CD+4) in solid neon matrices between 2.5 and 11 K: Analysis of tunneling. J. Chem. Phys. 1995, 103, 3377-3377.
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Knight, L. B.; Cobranchi, S. T.; Petty, J. T.; Cobranchi, D. P. The generation and electron spin resonance characterization of 63,65Cu12CH2 and 63,65Cu13CH2 in neon matrices. J. Chem. Phys. 1989, 91, 4587-4587.
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Knight, L. B.; Cobranchi, S. T.; Petty, J. T.; Earl, E. A.; Feller, D.; Davidson, E. R. Electron spin resonance investigations of 11B12C, 11B13C, and 10B12C in neon, argon, and krypton matrices at 4 K: Comparison with theoretical results. J. Chem. Phys. 1989, 90, 690-690.
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Knight, L. B.; Cobranchi, S. T.; Petty, J. T. Electronic ground state assignment for O+4 Neon matrix electron-spin resonance investigation. J. Chem. Phys. 1989, 91, 4423-4423.
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Knight, L. B.; Petty, J. T.; Cobranchi, S. T.; Feller, D.; Davidson, E. R. The generation of 12C31P and 13C31P by reactive laser vaporization for rare gas matrix electron spin resonance studies: Comparison with ab initio theoretical calculations. J. Chem. Phys. 1988, 88, 3441-3441.
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Knight, L. B.; Petty, J. T. Neon matrix ESR investigation of 69,71GaAs+ generated by the photoionization of laser vaporized GaAs(s). J. Chem. Phys. 1988, 88, 481-481.